Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. Invitrogen™ CholEsteryl 4,4-Difluoro-5- (4-Methoxyphenyl)-4-Bora-3a,4a-Diaza- s -Indacene-3-Undecanoate (CholEsteryl BODIPY™ 542/563 C 11 ) Orange-red fluorescent cholesteryl ester can be used as tracer of cholesterol transport and rand as general nonexchangeable membrane marker Manufacturer: Invitrogen™ C12680 Catalog No. Invitrogen™ Low Density Lipoprotein from Human Plasma, BODIPY™ FL ... Labelingmedium wasprepared throughmixing unlabeled cholesterol (0.025mM),BODIPY- cholesterol (0.1mM) andcyclo- Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Blood and BM cells were isolated and RBCs lysed as previously described . Bernecic NC, Zhang M, Gadella BM, Brouwers JFHM, Jansen JWA, Arkesteijn GJA, de Graaf SP, Leahy T. BODIPY-cholesterol can be reliably used to monitor cholesterol efflux from capacitating mammalian spermatozoa. AlexaFluor647 and DiI were obtained from Invitrogen; Atto647N-NHS was from Attotec. No. Invitrogen™ Low Density Lipoprotein from Human Plasma, BODIPY™ FL complex (BODIPY™ FL LDL) Nonacetylated conjugate Manufacturer: Invitrogen™ L3483 Catalog No. Lipids were solubilized at 65 °C in phos-phate-buffered saline by sonication (at 25% of full power setting) with a After cholesterol overload, the effect is analogous to that which has been reported in the case of Niemann-Pick type C (NPC) fibroblasts, where lac-Cer accumulates aberrantly in . . Results are based on five (endogenous cholesterol) six (BODIPY-cholesterol) independent samples and presented as the mean percent BODIPY-cholesterol or endogenous cholesterol remaining ± SEM. . Briefly, following dilution to 250 × 10 6 sperm/mL with TALP devoid of BSA and bicarbonate, spermatozoa were labelled with 1 µM BODIPY-cholesterol (1 mM stock solution . Invitrogen BODIPY® FL dye is a green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Alexa Fluor 488. BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza ... PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol ...
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